Journal: Oncogene

Article Title: Spermine oxidase promotes Helicobacter pylori -mediated gastric carcinogenesis through acrolein production

doi: 10.1038/s41388-024-03218-7

Figure Lengend Snippet: A Acrolein quantification by mass spectrometry in the gastric tissues of experimental animals; n = 3 per genotype. B Representative immunofluorescence images of acrolein adducts from WT and Smox –/– mice infected or not with H. pylori ; data are representative of n = 2 uninfected mice and n = 3 infected mice per genotype. AcroleinRED assay was performed on gastric organoids generated from naïve mice and infected or not ex vivo for 24 h with H. pylori ( C ) and the staining was quantified ( D ); color of symbol indicates organoids derived from same mouse but different passages/experiments, with n = 2 animals per genotype and n = 3 replicates per mouse. AGS cells pretreated or not with the SMOX inhibitor MDL 72527 (25 μM) for 24 h followed by infection or not with H. pylori for 24 h were then stained with AcroleinRED ( E ) and the staining was quantified ( F ). Human gastric organoids isolated from normal patient biopsies infected or not with H. pylori were stained with AcroleinRED ( G ) and the staining was quantified ( H ). I The antral gastric tissues from 3 normal and 5 patients with H. pylori ( Hp ) gastritis were immunostained for acrolein adducts. In all representative fluorescence images, AcroleinRED or acrolein adducts are depicted in red and nuclei are stained in blue with DAPI. Scale bars, 50 μm ( B and I , bottom row) or 100 μm (all other images). In all bar graphs, values are reported as mean ± SEM. Statistical analyses, where shown, * P < 0.05, **** P < 0.0001 determined by one-way ANOVA and Tukey test ( A , D , F ) or Student’s t test ( H ).

Article Snippet: All wells were thoroughly washed with PBS, fixed with 3.7% formaldehyde for 30 min at room temperature, washed with PBS, and mounted using the VECTASHIELD HardSet Antifade Mounting Medium with DAPI (Fisher Scientific).

Techniques: Mass Spectrometry, Immunofluorescence, Infection, Generated, Ex Vivo, Staining, Derivative Assay, Isolation, Fluorescence